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Wednesday 15 September |
| 10:00 - 13:00: Registration with welcome drinks + sponsor exhibitions |
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11:00 - 12:00: Presentations on subject "Trends in Food Safety Diagnostics". Organised and hosted by Prionics. |
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12:00 - 13:00: Lunch |
| 13:00 - 13:30: Opening speeches |
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13:30 - 17:15: First session: General session (including classical diagnostics) |
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13:30 - 14:15: Invited speaker
Dr. Christian Griot
Biorisk management in diagnostic laboratories; the future challenges
The laboratory biorisk management standard (CWA 15793) was developed by the world’s biosafety community with the objective to implement and promote the adoption of recognised standards for management of biological risk. The basis is continual improvement through a plan – do – check – act cycle as in other management standards. This approach enables an organization to effectively identify, monitor and control the laboratory biosafety and biosecurity of its activities. The implementation of this standard in already existing quality management systems will be a major challenge not only for diagnostic laboratories but also research facilities.
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14:15 - 15:05: Selected oral presentations
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Sam McCullough |
LEADDR: THE AUSTRALIAN EMERGENCY RESPONSE LABORATORY NETWORK |
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Caroline Frey |
INTESTINAL TRITRICHOMONAS FOETUS INFECTION IN CATS IN SWITZERLAND DETECTED BY IN VITRO CULTIVATION AND PCR |
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Nicola Pozzato |
EVALUATION OF A RAPID AND INEXPENSIVE LIQUID CULTURE SYSTEM FOR THE DETECTION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN BOVINE FAECES |
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Jens Böttcher |
DIAGNOSIS OF BHV1-INFECTION IN THE PRESENCE OF MATERNALLY DERIVED ANTIBODIES |
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15:05 - 15:35: Coffee break + poster sessions + sponsor exhibitions |
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15:35 - 17:15: Selected oral presentations
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Alex Raeber |
COMPARATIVE ANALYSIS OF THE BOVIGAM INTERFERON-GAMMA ELISA FOR BOVINE TUBERCULOSIS USING ALTERNATIVE ANTIGENS OR TUBERCULINS FOR BLOOD STIMULATION IN FIELD TRIALS AND EXPERIMENTAL INFECTIONS |
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Giulia Pezzoni |
EVALUATION OF ORF2 AND ORF3 RECOMBINANT ANTIGENS IN HEV ANTIBODY-DETECTION ELISA |
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Jens Böttcher |
UNEXPLAINED SINGLE-REACTORS IN BHV1-SEROLOGY |
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Britta Janowetz |
LONGITUDINAL TESTING OF SHEEP FOR COXIELLA BURNETII (PCR) AND PHASE I / PHASE II ANTIBODIES AFTER APPLICATION OF AN INACTIVATED PHASE I VACCINE |
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Awad Shehata |
RECOMBINANT POLYPEPTIDES AS ATOOL FOR IMPROVEMENT OF SEROLOGICAL DIAGNOSIS OF INFLUENZA A SUBTYPE H5 IN CHICKENS AND DUCKS |
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Gabriel Kovac |
BIOMARKERS OF METABOLIC DISORDERS IN PERIPARTAL PERIOD OF DAIRY COWS |
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Flavia Tisserant |
SEROLOGY AND MOLECULAR DIAGNOSTICS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS (EHDV) |
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Kees van Maanen |
PREDICTION OF SHEDDING OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS |
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17:15 - 18:15: General Meeting EAVLD (members EAVLD only) |
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17:15 - 18:15: Poster sessions + sponsor exhibitions (non-members) |
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18:15: End of first day, shuttle busses to hotel |
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19:00 - 21:00: Drink Reception at the Apollo Hotel in Lelystad. Organised and hosted by IDEXX. Reservation needed. |
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Thursday 16 September |
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09:00- 09:40: First session: General session (including classical diagnostics) (continuation) |
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09:00 - 09:40: Selected oral presentations
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Christina Tena |
FIRST HPAI H7 OUTBREAK IN POULTRY (LAYING HENS) IN SPAIN |
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Aymeric Hans |
EPIDEMIOLOGY AND VIRAL STRAINS CHARACTERIZATION OF THE 2009 EQUINE INFECTIOUS ANAEMIA FRENCH OUTBREAK |
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Barbara Bryce |
BESNOITIOSIS - AN EMERGING DISEASE IN EUROPEDEVELOPMENT OF A NEW TOOL FOR DISEASE SURVEILLANCE |
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| 09:40 - 12:45: Second session: Molecular diagnostics |
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09:40 - 10:25: Invited speaker
Dr. Phil Wakeley
Current molecular diagnostics and future prospects: making the tricky, routine, easy and reliable and then going outside.
The polymerase chain reaction is now standard in many veterinary diagnostic laboratories allowing rapid screening and characterisation of pathogens. Other isothermal methods in particular LAMP are also being used. These will be discussed with relevance to the challenges that still remain for both laboratory and point of need testing strategies.
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10:25 - 10:55: Coffee break + poster sessions + sponsor exhibitions |
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10:55 - 12:45: Selected oral presentations
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Peter Kirkland |
EVALUATION OF REAL TIME POLYMERASE CHAIN REACTION ASSAYS FOR THE DETECTION OF BLUETONGUE VIRUS IN BOVINE SEMEN. |
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Laura Valls Vila |
A NEW METHOLOLOGY FOR DETECTION AND QUANTIFICATION OF PATHOGENIC BACTERIA IN BOVINE BULK TANK MILK |
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John El-Attrache |
UTILIZATION OF VETMAX™- GOLD BVDV DETECTION KIT FOR THE DETECTION AND POTENTIAL DIFFERENTIATION OF PERSISTENTLY INFECTED AND TRANSIENTLY INFECTED CATTLE |
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Lise Grewis |
SENSITIVITY OF A REAL TIME PCR TEST FOR BOVINE TUBERCULOSIS DIAGNOSIS |
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Stéphan Zientara |
DEVELOPMENT OF MOLECULAR TOOLS FOR BTV AND EHDV TYPING IN THE FRENCH MARTINIQUE AND LA RÉUNION ISLANDS |
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Katja Ebert |
EVALUATION OF A PORTABLE AMPLIFICATION PLATFORM FOR LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) OF FOOT-AND-MOUTH DISEASE VIRUS (FMDV) AND AFRICAN SWINE FEVER (ASFV) |
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Charlotte Hjulsager |
DIAGNOSIS OF PORCINE RESPIRATORY DISEASE WITH A REAL-TIME PCR DIAGNOSTIC PACKAGE |
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Gerard Wellenberg |
PHYLOGENETIC ANALYSIS OF DUTCH PRRSV STRAINS |
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Giulia Pezzoni |
COMPARATIVE PERFORMANCE OF THREE GENOME AMPLIFICATION ASSAYS FOR DETECTION OF SWINE VESICULAR DISEASE VIRUS IN EXPERIMENTAL AND FIELD SAMPLES |
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12:45 - 14:00: Lunch + poster sessions + sponsor exhibitions |
| 14:00 - 14:50: Second session: Molecular diagnostics (continuation) |
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14:00 - 14:50: Selected oral presentations
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Ana Rocha |
SINGLE AND MULTIPLEX REAL TIME RT-PCR ASSAYS SPECIFIC FOR AHSV SEROTYPES 2, 4 AND 9 |
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Fabien Miszczak |
COMPARISON OF COMMERCIAL NUCLEIC ACID PURIFICATION AND REAL-TIME RT-PCR AMPLIFICATION KITS FOR THE DETECTION OF EQUINE ARTERITIS VIRUS IN EQUINE SEMEN USING TWO PREVIOUSLY DESCRIBED ONE-STEP REAL-TIME RT-PCR ASSAYS |
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Roger Maes |
LABORATORY DIAGNOSIS OF CORONAVIRUS INFECTIONS IN FERRETS |
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Gerard Wellenberg |
QUANTITATIVE TAQMAN REAL-TIME PCR TESTS FOR THE DETECTION OF MDV-1, RISPENS CVI988 AND HVT DNA IN WHITE BLOOD CELLS, ORGANS AND FEATHERS OF CHICKENS |
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14:50 - 17:30: Third session: Multiplex diagnostics (includes multiplex diagnostics with non-molecular tests, like antibody-based) |
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14:50 - 15:35: Invited speaker
Dr. Aart van Amerongen
Multi-analyte diagnostics: relevance and possibilities:
Multi-analyte testing enables the simultaneous detection of a broad range of target components including microorganisms. The pros and cons of such diagnostic tests will be discussed and several platforms will be shown.
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15:35 - 16:05: Coffee break + poster sessions + sponsor exhibitions |
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16:05 - 17:30: Selected oral presentations
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Kees van Maanen |
DEVELOPMENT AND VALIDATION OF A MULTI-PCR ASSAY, USING ONE EXTRACTION PROTOCOL AND HARMONISED REAL-TIME PCR PROTOCOLS FOR SENSITIVE DETECTION OF SEVEN DIFFERENT PATHOGENS INVOLVED IN ACUTE EQUINE UPPER RESPIRATORY TRACT DISEASE |
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Sandra Blome |
DIFFERENTIATING CLASSICAL SWINE FEVER VIRUS INFECTED FROM VACCINATED WILD BOAR USING A RECENTLY DEVELOPED MULTIPLEX REAL-TIME RT-PCR ASSAY |
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Claire Pelletier |
VALIDATION OF THREE MULTIPLEX REAL-TIME PCR FOR DIAGNOSIS OF MAJOR RESPIRATORY PATHOGENS IN CATTLE |
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Jason Sawyer |
EVALUATION OF LUMINEX MULTIPLEX ASSAY SYSTEM FOR ROUTINE VETERINARY SEROLOGY |
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John Clarke |
PERFORMANCE OF THE ENFERPLEX TB ASSAY IN GB CATTLE AND ASSESSMENT OF POTENTIAL SUITABILITY AS A DIVA TEST |
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Jo Mayers |
EVALUATION OF MESO SCALE DISCOVERY MULTIPLEX TECHNOLOGY FOR THE DETECTION OF ANTIBODIES TO RESPIRATORY DISEASES IN CATTLE |
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Jan Langeveld |
ANTIBODY AND ANTIGEN PROPERTIES IN PRION DETECTION OF RUMINANTS |
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17:45: End of second day, shuttle busses to diner location or hotel |
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18:00 - 24:00: Congress dinner (drinks and opportunity to visit museum, dinner starts at 19:30) |
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Friday 17 September |
| 09:00 - 12:45: Fourth session: Validation and accreditation |
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09:00 - 10:30: Invited speakers
Dr Matthias Greiner
Validation of laboratory tests: guidelines, pitfalls and statistics
Relevant guidelines for validation of diagnostic tests, including the stage model of the World Organisation for Animal Health (OIE) for certification of diagnostic assays will be presented. The emphasis will be on epidemiological issues arising when translating the fitness-for-purpose requirements of the OIE into the designing of validation studies. Special approaches for dealing with prohibitive costs or unavailability of a gold standard will be presented and discussed. Another practical issue is the choice of a cut-off value for quantitative diagnostic tests. Approaches for selecting a cut-off, which are related to the receiver operating characteristic (ROC) analysis and cut-off free methods for diagnostic inferences based on likelihood ratios will be demonstrated. There is also growing interest in systematic review and meta-analysis of diagnostic test studies. Therefore, the principles and some practical applications of these methods will be presented. In the same context, reporting guidelines for validation studies as well as guidelines for assessing study quality are relevant. These guidelines have been developed in the area of medicine and their applicability in veterinary diagnostics is to be discussed. The presentations will be illustrated using practically relevant examples.
Dr Russell Williams
Accreditation – defining and maintaining quality
What is quality? We have no instruments that can physically measure it or a scale to score it on, so is it just a matter of opinion? Accreditation is the way to ensure quality in laboratories and ISO 17025 is the defined world standard against which testing laboratories can be measured. In this presentation I will look at the processes by which accreditation is achieved and maintained. Also at aspects of the standard and how these apply in veterinary testing laboratories. We all learn from mistakes and so I’ll also be including examples of how not to go about things...
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10:30 - 11:00: Coffee break + poster sessions + sponsor exhibitions |
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11:00 - 12:45: Selected oral presentations
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Paula Johnson |
INTERLABORATORY COMPARISONS – WHY BOTHER? |
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Fabien Ramos |
EMERGENCE OF A NEW PANDEMIC INFLUENZAVIRUS (PH1N1) : DEVELOPMENT AND VALIDATION OF NEW PCR KITS FOR HIS DETECTION |
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Kees van Maanen |
VALIDATION OF A MULTISPECIES COMPETITION ELISA FOR DETECTION OF ANTIBODIES AGAINST INFLUENZA A VIRUSES IN SEVERAL AVIAN SPECIES, PIGS AND HORSES |
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Stephane de Moor |
DEVELOPMENT OF AN ELISA FOR DETECTION OF BOVINE PREGNANCY |
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Andrea Ballagi |
DETECTION OF ANTI-INFLUENZA A NUCLEOPROTEIN ANTIBODIES IN PIGS USING A COMMERCIAL INFLUENZA EPITOPE-BLOCKING ENZYME-LINKED IMMUNOABSORBENT ASSAY DEVELOPED FOR AVIAN SPECIES |
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Axel Colling |
THE NEW OIE PRINCIPLES AND METHODS FOR VALIDATION OF DIAGNOSTIC ASSAYS |
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Aymeric Hans |
EQUINE ARTERITIS VIRUS PROFICIENCY TEST FOR SEROLOGICAL AND VIROLOGICAL DIAGNOSIS IN EUROPE |
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Abdelfattah Monged Selim |
COMPARATIVE VALIDATION OF PCR-PROTOCOLS FOR DIRECT DETECTION OF MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN BOVINE FAECES |
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12:45 - 14:00: Lunch + poster sessions + sponsor exhibitions |
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14:00: End of congress, shuttle busses to train station Lelystad |
| The invited speakers |
Dr. Christian Griot, Institute of Virology and Immunoprophylaxis, Switzerland
Christian Griot (DVM, PhD) is a senior scientist and the Director of the Institute of Virology and Immunoprophylaxis (IVI), the Swiss National Reference Laboratory for Exotic Animal Diseases. The IVI with its BL-4 containment facility is responsible for the diagnosis of highly contagious animal disease such as foot and mouth disease, classical swine fever, bluetongue and avian influenza. As reference laboratory, the IVI conducts major research in these areas and participates in national and international competitive research programs. In addition, the IVI is responsible for the registration and licensing of all veterinary vaccines used in Switzerland and is fully accredited according to the ISO Norm 17025.
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Dr. Phil Wakeley, Veterinary Laboratories Agency, UK
Phil Wakeley has over 10 years experience working in the area of veterinary molecular diagnostics at the Veterinary Laboratories Agency - UK. In collaboration with colleagues he has developed, validated and instituted a suite of PCR and RT-PCRs over the years covering animal pathogens from rabies to contagious equine metritis. He has practical experience of using PCR in emergency response (CSF in 2000 in the UK) and more recently for avian influenza surveillance in wild birds. He is interested in point of need testing devices and is now actively pursuing projects combining isothermal amplification methods (LAMP, RPA) with simple detection methods.
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Dr. Aart van Amerongen, Wageningen University, The Netherlands
Dr. Aart van Amerongen studied Medical Biology at the University of Utrecht and did his PhD on a cholesterol and phospholipid transfer protein. Following a postdoc position at the Central Veterinary Institute in Lelystad, in which he applied synthetic peptides in mapping antibody epitopes, he joined the Agrotechnology & Food Sciences Group of Wageningen University and Research Centre some 20 years ago to coordinate the research in rapid, also multi-analyte diagnostic assays.
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Dr. Matthias Greiner, Federal Institute for Risk Assessment (BfR), Germany
Dr. Greiner (DVM, Dr. med. vet. habil, MSc Appl Stat, DipECVPH) graduated in Veterinary Medicine in 1986 and holds an MSc degree in Applied Statistics. He started his career as head of serological laboratories at the Department of International Animal Health (previously Tropical Veterinary Medicine and Epidemiology) and lecturer in veterinary epidemiology at the Freie Universität Berlin, Germany. He is Diplomat and Council Member of the European College of Veterinary Public Health (ECVPH).
From April 2002 to December 2005, he held a position as research professor and head of the Animal Health Section and the International EpiLab at the Danish Institute for Food and Veterinary Research in Copenhagen. During his leadership, the EpiLab has been accredited as OIE Collaborative Centre for Research and Training in Population Animal Health Diagnosis and Surveillance Systems and has gained international recognition for its research work related to this mandate.
In January 2006, Dr. Greiner joined the Federal Institute for Risk Assessment (BfR) in Berlin, Germany. His current scientific interest is diagnostic test validation, risk assessment and epidemiology in relation to microbiological food safety and toxicology. Dr. Greiner contributed to several working groups of the European Food Safety Authority (EFSA) and was a member of the Animal Health and Animal Welfare Panel (2006-2009).
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Dr. Russell Williams, Veterinary Laboratories Agency, UK
Russell Williams trained as a microbiologist, working in human and veterinary diagnostic testing, particularly in developing and validating PCR-based testing methods. He has worked in the Laboratory Services Department of the Veterinary Laboratories Agency (VLA) for the past ten years, based in Penrith in the Lake District. He is the Deputy Test Manager of the VLA ISO17025 Accreditation Group consisting of 24 testing laboratories and units spread over 16 sites. He is a consultant technical expert to the United Kingdom Accreditation Service (UKAS) on the accreditation of molecular test methods to ISO17025.
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